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miRNA Northern Blot Assays

Signosis’ miRNA High-Sensitivity Northern Blot Assay offers a non-radioactive, highly sensitive method for analyzing miRNA expression. Utilizing biotin-labeled detection probes and signal amplification, this assay delivers up to 100x greater sensitivity than traditional Northern blot methods—ideal for detecting both low/high abundance miRNAs.

It can also distinguish mature miRNAs from their precursors and is well-suited for validating PCR or microarray data. With strong customer feedback and numerous citations, this assay is a trusted tool for researchers investigating gene regulation and miRNA pathways worldwide.

Principle 

In this assay, RNA samples are first separated by gel electrophoresis and then transferred onto a membrane for blotting. To detect a specific miRNA, a biotin-labeled probe is used that hybridizes to the target miRNA sequence on the membrane.

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For moderate to high-expressing miRNAs, the regular Northern blot assay is sufficient. However, for low-abundance miRNAs, the Highly Sensitive Northern Blot Assay (NB-1001 and HP-XXXX) is recommended. This version uses a specially designed biotin-labeled probe that includes two components:

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  1. A miRNA-complementary sequence

  2. A tag sequence for signal amplification

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After hybridization, the tag sequence is recognized by a biotin-enriched amplifier, significantly boosting the detection signal. This enhanced sensitivity enables the detection of even low-level miRNA expression with precision and reliability.

Assay
Product
SKU
Price (USD$)
Northern Blot

miRNA Northern Blot Assay Kit without gels

NB-0002
481
Northern Blot

Highly Sensitive miRNA Northern Blot Assay Kit without gels

NB-1002
511

Products

Benefits

Non-Radioactive Design

Safe and easy-to-use biotin-labeled probes eliminate the need for radioactive components.

​Streamlined Workflow

Pre-labeled probes simplify the process, saving time and effort.

High Sensitivity

Detect low-abundance miRNAs with 100x more sensitivity than traditional methods with an amplifier.

Validation Tool

​Ideal for validating miRNA expression results from PCR or other methods, ensuring accuracy and reliability.

​Comprehensive Analysis

Quantify both mature and precursor miRNAs with precise size determination.

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