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miRNA Real-Time PCR

Signosis’ miRNA Real-Time PCR Kit enables direct quantification of miRNA levels from total RNA or cell lysates—without the need for cDNA conversion. The kit is designed to process up to 12 samples and supports up to 50 real-time PCR reactions, offering a streamlined and efficient workflow for miRNA expression analysis.

Principle 

In this assay, the target miRNA is first hybridized with two specially designed DNA oligonucleotides to form an RNA/DNA duplex. When the sequences are perfectly matched, the oligos align precisely with the miRNA, allowing a DNA ligase to join them together.

 

A single nucleotide difference in the miRNA sequence can prevent either the hybridization or the ligation step, ensuring high specificity. Once ligated, the joined DNA molecule serves as the template for real-time PCR, enabling sensitive and specific quantification of the target miRNA.

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Products

Assay
Product
SKU
Price (USD$)
Real-Time PCR

miRNA Real-Time PCR Assay Kit

CL-0004
550
Real-Time PCR

miR-17-92 Cluster Real-Time PCR Assay Kit

CL-0005
709
Real-Time PCR

Let-7 Isoform Real-Time PCR Assay Kit

CL-0006
709

Benefits

Convenient

Reagents needed for cell lysis and PCR are included in the kit

No RNA Preparation

Cell lysates can be used directly in reverse transcription, as well as total RNA.

Real-Time

Analysis can be monitored in real time.

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