miRNA Real Time PCR

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miRNA Real Time PCR

With the miRNA Real-Time PCR kits, miRNA can be analyzed from total RNA or from cell lysates directly without cDNA conversion. This kit can be used for 12 samples and 50 PCR reactions.

Principle

In the assay, the target miRNA molecule is hybridized with two oligos to form a RNA/DNA duplex. When the sequences are perfectly matched, they are aligned with the miRNA and the joint can be ligated with DNA ligase. A single nucleotide difference among miRNAs will block either the hybridization or the ligation. After the pair of oligos is ligated, the ligated molecules are subjected to real-time PCR analysis.

Benefits

Convenient

All reagents from cell lysis to PCR, are included in one kit

No RNA Preparation

Cell lysates can be used directly in reverse transcription, although total RNA is perfectly fine.

Real-Time

Analysis can be monitored in real time.

Products

$550.00
$675.00
- let-7a
- let-7b
- let-7c
- let-7d
- let-7e
- let-7f
- let-7g
- let-7i
- miR-1
- miR-100
- miR-101-1
- miR-103
- miR-106a
- miR-106b
- miR-107
- miR-10a
- miR-122a
- miR-124a
- miR-125a
- miR-125b
- miR-126
- miR-127
- miR-128a
- miR-128b
- miR-131
- miR-132
- miR-133a
- miR-133b
- miR-134
- miR-135b
- miR-136
- miR-137
- miR-140
- miR-141
- miR-142-3p
- miR-142-5p
- miR-143
- miR-144
- miR-145
- miR-146a
- miR-146b
- miR-148a
- miR-149
- miR-150
- miR-151
- miR-152
- miR-153
- miR-154
- miR-155
- miR-15a
- miR-16
- miR-17-3p
- miR-17-5p
- miR-181a
- miR-181b
- miR-181c
- miR-181d
- miR-182
- miR-183
- miR-184
- miR-185
- miR-186
- miR-188
- miR-18a
- miR-190
- miR-191
- miR-192
- miR-193a-3p
- miR-193a-5p
- miR-194
- miR-195
- miR-196a
- miR-196b
- miR-197
- miR-19a
- miR-20a
- miR-21
- miR-22
- miR-23a
- miR-24
- miR-25
- miR-26a
- miR-27a
- miR-28
- miR-29a
- miR-29b
- miR-29c
- miR-30a-3p
- miR-30a-5p
- miR-30b
- miR-30c
- miR-31
- miR-7
- miR-9
- miR-92
- miR-92b
- miR-93
- miR-95
- miR-96
- miR-99a