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miRNA Real Time PCR

With the miRNA Real-Time PCR kits, miRNA can be analyzed from total RNA or from cell lysates directly without cDNA conversion. This kit can be used for 12 samples and 50 PCR reactions. 


In the assay, the target miRNA molecule is hybridized with two oligos to form a RNA/DNA duplex. When the sequences are perfectly matched, they are aligned with the miRNA and the joint can be ligated with DNA ligase. A single nucleotide difference among miRNAs will block either the hybridization or the ligation. After the pair of oligos is ligated, the ligated molecules are subjected to real-time PCR analysis.



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