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100+ non-radioactive EMSA Kits are available. The probes are pre-labeled with biotin, and all reagents are included in the kit. 


When one TF is activated, it binds to a biotin-labeled probe, which is designed based on the consensus binding sequence of the TF. When the formed complex is subjected to electrophoresis, it runs slower than the free probe, and can be easily distinguished. Two or more samples can be compared and differences can be determined by the density of the shifted band.

EMSA diagram 092815.png


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