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Mouse Tumor Marker ELISA Kits


Autoimmune ELISA kits measure autoimmune antibodies in the serum. It is based on the principle of a solid phase enzyme-linked immunosorbent assay. The assay utilizes a specific antigen for immobilization on the microtiter wells and anti-mouse IgG antibody conjugated to horseradish peroxidase (HRP) for detection. The test sample is allowed to react simultaneously with the two components, resulting in autoimmune antibodies being sandwiched between the solid phase antigen and the enzyme-linked antibodies. After incubation, the wells are washed to remove unbound antibodies. Then an HRP substrate is added to develop a blue color. The color development is stopped with the addition of Stop Solution, changing the color to yellow. The concentration of autoimmune antibodies is directly proportional to the color intensity of the test sample. Absorbance is measured spectrophotometrically at 450 nm.

Autoimmune ELISA diagram.gif

Tumor markers are substances found in the body that are produced by cancer cells or healthy cells in response to cancer. These markers provide important information because they can be used to help detect the presence of a cancer during diagnosis and determine the best type of treatment for the cancer. In addition, cancer biomarkers are used by physicians to monitor how a cancer is progressing and responding to a certain treatment. Signosis provides a series of tumor marker ELISA kits, including CA125, CTSD, CEA, E-selectin, and galectin-3.


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