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Snapshot Magnetic Beads
ChIP Assays

Capture real-time transcription factor interactions at the promoter level using only 10⁶ cells. The Snapshot Bead Assay allows precise analysis of TF complexes—including coactivators and corepressors—bound to specific promoters, offering powerful insight into gene regulation dynamics in live cells.

Principle 

Signosis' Magnetic Beads ChIP Assay improves traditional ChIP by enabling more efficient and sensitive detection of transcription factor complexes. The assay involves four steps: (1) cross-linking proteins to DNA, (2) chromatin fragmentation, (3) immunoprecipitation, and (4) target identification.

Key improvements include:

  • Dual cross-linking: Combines formaldehyde (short spacer arm) with a second reagent (long spacer arm) to capture both DNA-bound TFs and associated coactivators/corepressors.

  • MNase digestion: Efficiently fragments chromatin into mononucleosomes (~500 bp), enhancing sensitivity and reproducibility.

  • Streamlined DNA recovery: Uses a one-step Chelex-100 method to release DNA, eliminating proteinase K digestion and extra purification steps.

These enhancements make the assay faster, more reliable, and ideal for studying protein-DNA interactions in detail.

ChIP sequencing (2)_edited.jpg

Benefits

Precision

DNA is cleaved using MNase digestion instead of sonication to produce more uniform DNA fragments.

High Specificity

The cross-linked protein-DNA complexes are pulled down by a specific antibody and ChIP grade protein A/G magnetic beads.

Convenience

Cross-linked DNA is released using a one-step Chelex-100 treatment instead of the tedious digestion and column purification method.

User-Friendly

Streamlined protocol with ready to use reagents simplify the execution of the experiment.

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