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NF-κB is a critical regulator of inflammatory responses, proliferation and differentiation of T-cells. The aberrant activation of NFkB  can contribute to the development of autoimmunity, chronic inflammation, or lymphoid cancer.  Jurkat cells are human T lymphocyte cells widely used to study T cell signaling.  Signosis developed a stable Jurkat NFkB-luciferase reporter stable cell line, which can be used for easily monitoring the activation of NFkB activation in T cells through sensitive luciferase analysis. This cell line was established by transfection using a pTA-NFkB-luciferase reporter vector, along with hygromycin expression vector followed by hygromycin selection.  The hygromycin resistant clones were subsequently screened for luciferase activity induced by TNFapha treatment.



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