Principle
NF-κB is a critical regulator of inflammatory responses, proliferation and differentiation of T-cells. The aberrant activation of NFkB can contribute to the development of autoimmunity, chronic inflammation, or lymphoid cancer. Jurkat cells are human T lymphocyte cells widely used to study T cell signaling. Signosis developed a stable Jurkat NFkB-luciferase reporter stable cell line, which can be used for easily monitoring the activation of NFkB activation in T cells through sensitive luciferase analysis. This cell line was established by transfection using a pTA-NFkB-luciferase reporter vector, along with hygromycin expression vector followed by hygromycin selection. The hygromycin resistant clones were subsequently screened for luciferase activity induced by TNFapha treatment.
Inflammation/NFKB
Introduction
The NF-κB signaling pathway is a key regulator of inflammation, immune response, and cell survival, controlling the transcription of pro-inflammatory cytokines and stress-response genes. Aberrant NF-κB activation is strongly associated with chronic inflammation, autoimmune diseases, cancer, and neurodegenerative disorders, making it a high-value target in inflammation research and therapeutic development.
Our NF-κB luciferase reporter assays and NF-κB reporter stable cell lines provide a high-sensitivity NF-κB assay for quantitative measurement of pathway activation. These luciferase reporter systems enable reliable detection of NF-κB activity induced by inflammatory cytokines, GPCR signaling, Toll-like receptor agonists, and small-molecule compounds.
Designed for inflammation-focused drug screening, these NF-κB assays deliver high signal-to-background ratios, excellent reproducibility, and compatibility with high-throughput screening (HTS). They are ideally suited for mechanistic studies, compound profiling, and inhibitor discovery in academic research and pharmaceutical drug discovery programs.
Products
Firefly Luciferase Reporter Stable Cell Lines
Signosis' cell line for monitoring NFkB binding and activation using the standard Firefly Luciferase as the reporter.
Secreted Luciferase Reporter Stable Cell Lines
Secreted Luciferase Reporter Stable Cell lines provide distinct advantages over traditional Firefly luciferase reporters.
Because secreted luciferases are released into the culture medium, they enable noninvasive, real-time monitoring of gene activity. This allows researchers to follow long-term, continuous changes in the same cell population, while eliminating the need for cell lysis and streamlining experimental workflows.
