Metabolic Stress
Principle
Besides the central role of amino acids as building blocks of proteins they are also involved in other cellular functions like transcription, mRNA maturation, translation, mRNA turnover, autophagy and metabolism. Lack of amino acids (deprivation) will trigger the amino acid response (AAR) pathway largely through signal transduction and mediated through altered transcriptional activity of specific genes, including eIF2α, Asns, Atf4 and Chop.
Signosis has developed a luciferase reporter stable cell line under control of AARE (Amino acid response element) that can be easily used to monitor activation of Amino acid response Signaling Pathway.
Benefits
High Sensitivity & Responsiveness
Each cell line is validated to induce a strong reporter signal in response to stimuli.
Consistent
TF reporter construct is stably integrated into the genome to avoid experimental/cell-to-cell variations.
Time-Saving
Cell line can be used for experiments right away to study different signaling pathways.
Routine Mycoplasma Testing
All cell lines tested negative for mycoplasma.
