Androgen Receptor
Principle
The androgen receptor (AR) plays a critical role in reproductive function, muscle growth, and is implicated in conditions such as prostate cancer. Upon activation by specific stimuli, AR translocates from the cytoplasm to the nucleus, where it binds to androgen response elements on DNA to regulate gene expression. Signosis has developed a stable AR luciferase reporter cell line in which luciferase activity directly reflects AR activation. This cell line serves as a robust reporter system for monitoring AR activation triggered by stimuli, gene overexpression, or gene knockdown.
The stable cell line was established by co-transfecting an AR luciferase reporter vector and a G418 resistance vector, followed by antibiotic selection. G418-resistant clones were screened for etoposide-induced luciferase activity, and the clone stably showing the highest induction was selected and expanded for our cell lines.
Benefits
High Sensitivity and Responsiveness
Each cell line is validated to induce a strong reporter signal in response to stimuli.
Consistent
The TF reporter construct is stably integrated into the genome to avoid cell-to-cell variations in experiments.
Efficient
Cell lines can be used right away for experiments to study different signaling pathways.
Mycoplasma Free
All cell lines have been tested negative for mycoplasma.
