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Androgen Receptor


The androgen receptor (AR) plays a crucial role in the development of male reproductive organs. When activated by stimuli, AR translocates from the cytoplasm into the nucleus, which then binds a DNA recognition site and regulates gene expression. Signosis has established an AR luciferase reporter stable cell line, in which luciferase activity is specifically associated with the activity of AR. Therefore, the cell line can be used as a reporter system for monitoring the activation of AR triggered by stimuli treatment, enforced gene expression, and/or gene knockdown.


The cell line was established by transfection of an AR luciferase reporter vector along with G418 expression vector followed by G418 selection. The G418 resistant clones were subsequently screened for etoposide-induced luciferase activity. The clone with the highest fold induction (22 fold) was selected and expanded to produce this stable cell line.



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