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Gene-Specific Promoter Luciferase Reporter vectors

Promoter-based luciferase reporters provide a powerful, biologically relevant way to study transcriptional regulation and pathway activity. By placing the luciferase gene under the control of native gene promoter regions, these constructs capture the natural arrangement of transcription factor sites, cofactors, and regulatory elements, delivering readouts that closely mirror endogenous gene expression rather than simplified synthetic motifs.

Principle 

Our promoter driven reporter collection covers a broad panel of pathway and disease relevant genes, including ICAM1, SOCS3, VEGFA, CDKN1A (p21), HMOX1 (HO-1), PAI 1 (SERPINE1), HES1, AXIN2, TFF1 (PS2), GILZ, SLC2A1, CCL2, COX2, MMP9, and IL2, enabling you to monitor inflammation, hypoxia/oxidative stress, NF κB and JAK/STAT signaling, Wnt/Notch pathways, glucocorticoid responses, and more within a native regulatory context. Unlike reporters built from repeated consensus binding sites, these promoter-based constructs integrate multiple upstream inputs and chromatin effects, improving specificity, reducing artifacts from promiscuous motifs, and providing transcriptional responses that more faithfully predict endogenous mRNA changes for each gene.

With low background, robust inducibility, and compatibility with minimal promoters and stable cell line generation, our promoter-based luciferase vectors are ideal for drug screening, mechanism-of-action studies, biomarker validation, and pathway profiling in disease-relevant cell models.

Benefits

  • Native promoters from ICAM1, SOCS3, VEGFA, CDKN1A (p21), HMOX1, PAI‑1, HES1, AXIN2, TFF1, GILZ, SLC2A1, CCL2, COX2, MMP9, and IL2 drive reporter expression that closely reflects endogenous gene regulation.
  • Biologically relevant signals: Reporter activity reflects endogenous control by key pathways, including inflammation, hypoxia/oxidative stress, Wnt/Notch, JAK/STAT, NF‑κB, and glucocorticoid signaling.
  • Fewer false positives: The intact promoter context reduces artefacts from promiscuous multimerized motifs and better tracks genuine pathway engagement.
  • Optimized assay performance: Native promoters combined with minimal promoters and stable integration give low background, strong induction, and high signal‑to‑noise for HTS and dose–response studies.
  • Custom options: Custom promoter-based luciferase reporters can be built for any promoter of interest to create pathway or gene specific assays tailored to your targets and cell models.
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