WNT/β-Catenin Pathway
The Wnt/β-Catenin signaling pathway is a key regulator of embryonic development, cell proliferation, stem cell pluripotency, and tumorigenesis. Upon activation by Wnt ligands, stabilized β-catenin translocates to the nucleus and partners with TCF/LEF transcription factors to drive the expression of critical downstream target genes.
Signosis offers a comprehensive suite of validated TCF/LEF Luciferase Reporter and Wnt1 Overexpression Stable Cell Lines engineered to monitor, stimulate, and dissect Wnt pathway dynamics in live cells:
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TCF/LEF Reporter Cell Lines (CHO-K1, HEK293, HeLa): Feature a reporter vector with 6 repeats of TCF/LEF binding sites driving firefly luciferase expression, enabling highly sensitive, quantitative measurement of β-catenin-dependent transcriptional activation in response to extracellular stimuli, gene knockdowns, or small-molecule modulators.
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Wnt1 Overexpression HEK293 Cell Line: Stably overexpresses full-length human Wnt1 ligand, serving as a reliable endogenous source of active Wnt1 for paracrine signaling studies, co-culture experiments, and direct pathway activation.
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Autocrine Wnt1/TCF-Luciferase Cell Line: Combines constitutive Wnt1 ligand production with TCF/LEF reporter readouts, establishing a self-activating autocrine signaling model ideal for high-throughput screening of Wnt/β-catenin antagonists without requiring exogenous ligand addition.
These robust, ready-to-use stable cell lines provide flexible experimental tools for drug discovery, oncology research, stem cell maintenance, and developmental signaling studies.
Stable Cell Lines Products
TCF/LEF Luciferase Reporter Stable Cell Lines
Name | SKU | Formatted Price |
|---|---|---|
Wnt1 overexpression HEK293 stable cell line | SL-6004 | $2,100.00 |
Wnt1 overexpression Stable Cell Line
Autocrine Wnt1-TCF/LEF Luciferase Reporter Stable Cell Line
Name | SKU | Formatted Price |
|---|---|---|
Wnt1-TCF/LEF Luciferase Reporter HEK293 Stable Cell Line | SL-6005 | $3,870.00 |
Benefits
High Sensitivity & Responsiveness
Each cell line is validated to induce a strong reporter signal in response to stimuli.
Consistent
TF reporter construct is stably integrated into the genome to avoid experimental/cell-to-cell variations.
Time-Saving
Cell line can be used for experiments right away to study different signaling pathways.
Routine Mycoplasma Testing
All cell lines tested negative for mycoplasma.
