Environmental Stress & Toxicology Signaling Stable TF Reporter Cell Panel
Introduction
The Environmental Stress & Toxicology Panel is a ready-to-use 96-well plate pre-seeded with luciferase reporter stable cell lines targeting up to eight key environmental stress and toxicology pathways.
Designed for toxicity profiling, xenobiotic metabolism studies, and hypoxia research, this panel enables researchers to analyze multiple stress-related signaling networks in a single, standardized assay format.
Our One-Step Cell Panel plate comes pre-loaded with stable TF reporter cells and is ready to use—no cell seeding or culturing required. Simply add your treatments and measure luciferase activity.
This assay utilizes mammalian cells to monitor the activation of eight critical transcription factor (TF) signaling pathways: XRE, FXR, NRF2, HIF, p53, NFκB, ATF4, and CREB. Each row of the 96-well plate contains cells transfected with a luciferase reporter plasmid under the control of a specific TF-responsive element, enabling simultaneous analysis of eight distinct signaling pathways across 12 different treatments.
Upon stimulation, activated transcription factors bind to their corresponding response elements within the reporter construct, inducing the expression of firefly luciferase. The level of luciferase activity, quantified using a luciferase substrate in the provided lysis buffer, directly reflects the activation status of the respective pathway.
Application
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Profiling xenobiotic metabolism and activation of detoxification pathways mediated by TFs like XRE and FXR.
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Investigating oxidative stress responses through NRF2 signaling under toxic or stressful conditions.
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Studying cellular adaptation to hypoxia using HIF reporter activity.
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Assessing DNA damage and genotoxic stress via p53 activation.
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Evaluating inflammatory and stress signaling pathways involving NF-κB, ATF4, and CREB.
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Toxicity screening of environmental pollutants, pharmaceuticals, and industrial chemicals for safety and risk assessment.
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Mechanistic studies of environmental stress-induced cellular dysfunction and disease processes.
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Supporting drug discovery efforts targeting pathways involved in cellular stress and toxicity.
