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Inflammation & Immune Signaling
Response Stable Cell Panel

Introduction

The culture-free Inflammation & Immune Signaling Panel is a ready-to-test 96-well plate pre-seeded with luciferase reporter stable cell lines targeting eight key immune and inflammatory pathways, including NFκB, NFAT, NRF2, AP-1, CREB, STAT1, STAT3, ISRE, and IRF. Designed for drug discovery and pathway profiling, this panel eliminates the need for cell seeding or culturing. Each well contains validated, stable reporter cell lines, enabling researchers to perform a single-step assay—just add your treatments and measure luciferase activity. In addition to these core immune pathways, the panel can be customized to include other transcription factors of interest, providing a flexible and standardized solution for immunology and inflammation research.

Principle

The One-Step Inflammation & Immune Signaling Panel uses luciferase reporter stable cell lines to monitor the activation of nine transcription factor (TF) pathways involved in immune regulation and inflammatory signaling. Each reporter cell line contains a luciferase gene under the control of a TF-responsive element. When activated, the TF binds to its response element, inducing luciferase expression that is quantified as luminescence.

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  • NFκB (Nuclear Factor Kappa B): Detects pro-inflammatory signaling pathways involved in cytokine production, immune response, and cell survival.

  • NFAT (Nuclear Factor of Activated T Cells): Monitors T-cell receptor–mediated transcription essential for immune cell activation and differentiation.

  • NRF2 (Nuclear Factor Erythroid 2–Related Factor 2): Reflects antioxidant defense and cellular protection mechanisms during inflammation and oxidative stress.

  • AP-1 (Activator Protein 1): Tracks stress- and cytokine-driven transcription linked to inflammation, proliferation, and immune response.

  • CREB (cAMP Response Element-Binding Protein): Reports on cAMP-related signaling involved in immune regulation and inflammatory mediator expression.

  • STAT1 (Signal Transducer and Activator of Transcription 1): Detects interferon-driven transcription important for antiviral immunity and inflammatory responses.

  • STAT3 (Signal Transducer and Activator of Transcription 3): Monitors cytokine and growth factor signaling associated with immune regulation and inflammation resolution.

  • ISRE (Interferon-Stimulated Response Element): Measures type I interferon response and antiviral gene activation.

  • IRF (Interferon Regulatory Factor): Reflects transcriptional activity of interferon regulatory proteins involved in antiviral defense and immune modulation.

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Application

  • Profiling Toll-like receptor (TLR) signaling pathways to dissect innate immune responses and pathogen recognition mechanisms

  • Investigating cytokine storm dynamics and hyperinflammatory responses relevant to infectious diseases and sepsis

  • Studying the molecular and cellular mechanisms driving autoimmune diseases through dysregulated cytokine and TLR signaling

  • Screening and evaluating immunomodulatory drugs targeting cytokine production and TLR-mediated pathways

  • Biomarker discovery and therapeutic target identification for managing excessive inflammation and autoimmunity

  • Functional characterization of immune signaling cascades for vaccine adjuvant development and immune therapy optimization

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Benefits

Stable Cell Line Consistency

Built with validated, stable luciferase reporter cell lines to ensure high reproducibility and minimize experimental variability.

True One-Step Workflow

​Pre-seeded, stable reporter cell lines eliminate cell seeding, culturing, and transfection—simply add your treatments and measure luciferase activity.

Comprehensive Pathway Profiling

Simultaneously analyze 8 key transcription factor (TF) pathways in a single 96-well format for efficient pathway screening.

Complete Reagent Package

Includes lysis buffer and luciferase substrate

Validated, Ready-to-Assay Cells

Each well contains quality-controlled reporter cells with consistent pathway responsiveness, removing the need for assay optimization.

Flexible Experimental Design

Supports up to 12 treatment conditions across all pathways in one experiment, enabling high-throughput, comparative analysis.

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