The Inflammation & Immune Signaling Panel is a ready-to-use 96-well plate pre-seeded with luciferase reporter stable cell lines targeting up to eight key immune and inflammatory pathways. Designed for drug discovery and pathway profiling, this panel enables researchers to analyze multiple signaling networks in a single, standardized assay format.

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Our One-Step Cell Panel plate comes pre-loaded with stable TF reporter cells and is ready to use -- no cell seeding or culturing. Simply add your treatments and measure luciferase activity.

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This assay utilizes mammalian cells to monitor the activation of eight key transcription factor (TF) signaling pathways: NFκB, NFAT, NRF2, AP-1, CREB, STAT1, STAT3, ISRE, and IRF. Each row of the 96-well plate contains cells transfected with a luciferase reporter plasmid under the control of a specific TF-responsive element, enabling simultaneous analysis of eight distinct signaling pathways across 12 different treatments.

 

Upon stimulation, activated transcription factors bind to their corresponding response elements within the reporter construct, inducing the expression of firefly luciferase. The level of luciferase activity, quantified using a luciferase substrate in the provided lysis buffer, directly reflects the activation status of the respective pathway.

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