Culture-Free Metabolism
Cell Panel Assay
Introduction
The culture-free Metabolic TF Reporter Panel is a ready-to-use 96-well plate pre-seeded with luciferase reporter stable cell lines targeting eight key transcription factor pathways: PPAR, FXR, CREB, FOXO3, STAT1, STAT3, TGFβ, and Wnt. The Metabolic Signaling Response Panel quickly profiles lipid metabolism, glucose regulation, and nutrient sensing, making it ideal for high-throughput metabolic pathway analysis.
Designed for convenience, it eliminates the need for cell seeding or culturing—just add your compounds and measure luciferase activity.
The panel can also be customized with additional transcription factors, offering a flexible, standardized solution for drug discovery, nutrient sensing studies, and metabolic signaling research.
Please review our full range of TF stable cell lines used in these panel assays for your reference.
How it works
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Pre-seeded ready to go: Each well contains a TF-luciferase reporter stable cell line—4 °C-preserved and pre-validated.
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Run your assay: Simply add compounds, incubate, and read luminescence signals.
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Read out: TF activation → DNA binding → luciferase expression → quantifiable luminescence.
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Included Stable cell lines
Benefits
Culture-Free & Ready-to-Use
Pre‑seeded with validated luciferase reporter cell lines; no cell culture facilities needed.
Fast, One‑Step Workflow
Just add compounds and measure luminescence for rapid results.
Standardized & Reproducible
Consistent assay conditions minimize variability between runs.
Broad Accessibility
Usable by any lab, regardless of cell culture expertise or infrastructure.
High‑Throughput Capable
96‑well format for efficient screening of large compound libraries.
Complete Reagent Package
Includes lysis buffer and luciferase substrate
Comprehensive Pathway Coverage
Monitors 8 core metabolic TF pathways
Highly Sensitive & Quantitative
Direct correlation between TF activation and luminescence signal
Customizable
Add transcription factors to fit your specific research goals.