RNA EMSA
RNA-binding proteins (RBPs) are essential regulators of gene expression at the post-transcriptional level. They play critical roles in processes such as mRNA splicing, stability, localization, translation, and the processing of non-coding RNAs including miRNAs and lncRNAs. To study these interactions, EMSA (Electrophoretic Mobility Shift Assay) provides a fast, reliable method for detecting the direct binding between RBPs and their RNA or DNA targets. By observing mobility shifts in a native gel, EMSA reveals binding specificity, affinity, and complex formation. This makes it a powerful tool for functional genomics, pathway analysis, and the characterization of RNA-binding proteins involved in key regulatory mechanisms and disease pathways.
Principle
RNA EMSA (Electrophoretic Mobility Shift Assay) is a sensitive technique used to study RNA–protein interactions by observing shifts in electrophoretic mobility. In this assay, a biotin-labeled RNA probe—prepared by in vitro transcription—is incubated with an RNA-binding protein or cell extract. To enhance specificity, especially when studying RNA-binding proteins with similar sequence preferences, longer RNA probes (100 nt) corresponding to functional promoter or regulatory regions are used. These extended probes allow for more accurate detection of protein–RNA binding under near-physiological conditions. Upon binding, the RNA–protein complex migrates more slowly through a native polyacrylamide gel compared to free RNA. After electrophoresis, the RNA is transferred to a membrane and detected via chemiluminescence using a streptavidin–HRP system. RNA EMSA enables precise analysis of RNA-binding activity, specificity, and interaction strength.
Products
Product Name | Price | SKU# |
|---|---|---|
hnRNP A1 RNA EMSA Kit | $535.00 | |
FUS RNA EMSA Kit | $535.00 | |
AUF1 RNA EMSA Kit | $535.00 | |
Poly(A)-Binding Protein (PABP) RNA EMSA Kit | $535.00 | |
Tristetraprolin RNA EMSA Kit | $535.00 | |
IRE RNA EMSA Kit | $535.00 | |
EMSA Without Probe | $508.00 | |
HuR RNA EMSA | $535.00 |
Benefits
High Specificity
Longer RNA probes (100 nt) improve discrimination between similar RNA-binding proteins by preserving native structural and sequence context.
Fast & Simple Workflow
Can be completed in a day with minimal material and no specialized equipment.
Direct Binding Evidence
Detects physical interaction between RNA and protein without the need for secondary readouts.
Non-Radioactive
Uses biotin labeling for safe, stable, and chemiluminescent detection—no radioactive handling required.
Quantitative & Sensitive
Allows detection of binding strength and affinity using biotin–streptavidin chemiluminescence.
Versatile Probe Design
In vitro transcription enables custom probe synthesis from functional regulatory regions.