RNA-Protein EMSA Assay Kits
Clean Visual Validation of RNA-Protein Interactions
RNA-binding proteins (RBPs) are essential regulators of gene expression, controlling mRNA splicing, stability, and non-coding RNA processing (miRNAs/lncRNAs). Dysregulation of these pathways drives numerous diseases, making RBP validation vital for functional genomics and target discovery.
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The Electrophoretic Mobility Shift Assay (EMSA) is the gold standard for verifying direct RBP-RNA interactions. Running complexes on a native gel provides clear visual validation of binding specificity, affinity, and multi-protein assembly.
Principle
The assay is based on the fact that RNA-protein complexes migrate more slowly through a non-denaturing polyacrylamide gel than free RNA probes.
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Binding Reaction: A biotin-labeled RNA probe is incubated with a purified protein or nuclear/cytoplasmic extract.
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Electrophoresis: The mixture is resolved on a native gel. Free probes migrate quickly to the bottom, while bound RNA-protein complexes are retarded ("shifted") higher in the gel.
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Detection: The gel is transferred to a membrane and visualized using an optimized, ultra-sensitive chemiluminescent substrate.
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Specificity Control: Specificity is confirmed by adding an excess of unlabeled ("cold") competitor probe, which prevents the formation of the shifted biotin-labeled complex.
RNA EMSA Kits
Name | SKU | Price (USD) |
|---|---|---|
HuR (ELAVL1) RNA EMSA Kit | GR-0001 | $749.00 |
AUF1 (HNRNPD) RNA EMSA Kit | GR-0005 | $749.00 |
TTP (ZFP36) RNA EMSA Kit | GR-0008 | $749.00 |
CELF1 (CUGBP1) RNA EMSA Kit | GR-0009 | $749.00 |
IRP1 RNA EMSA Kit (IRE) | GR-0010 | $749.00 |
SLBP RNA EMSA Kit | GR-0011 | $749.00 |
IRP2 RNA EMSA Kit (IRE) | GR-0012 | $749.00 |
HCV IRES–eIF3 RNA EMSA Kit | GR-0013 | $749.00 |
PTBP1 (hnRNP I) RNA EMSA Kit | GR-0014 | $749.00 |
TDP-43 (TARDBP) RNA EMSA Kit | GR-0015 | $749.00 |
SRSF1 (ASF/SF2) RNA EMSA Kit | GR-0016 | $749.00 |
NOVA1/NOVA2 RNA EMSA Kit | GR-0017 | $749.00 |
LIN28A RNA EMSA Kit (let-7 Loop) | GR-0018 | $749.00 |
Staufen RNA EMSA Kit | GR-0019 | $749.00 |
Smaug RNA EMSA Kit | GR-0020 | $749.00 |
Pumilio (PUM1/2) RNA EMSA Kit | GR-0021 | $749.00 |
Benefits
Universal Compatibility
Validated for human, mouse, rat, and all other mammalian samples—ideal for translational research.
Safe, Non-Radioactive
Matches traditional radioactive sensitivity while eliminating regulatory burdens, hazards, and disposal costs.
Pre-Validated
Probe Pairs
Includes a biotin-labeled "hot" probe and an unlabeled "cold" competitor, enabling immediate specificity controls without oligo design.
Comprehensive Insights
Easily characterize binding dynamics, measure competition, and visualize multi-protein supershifts.
Ultra-Sensitive Chemiluminescence
Combining an optimized workflow with a highly sensitive substrate ensures clear detection of low-abundance RBPs and weak interactions.
