RNA EMSA Assay Kits
Clean Visual Validation of RNA-Protein Interactions
RNA-binding proteins (RBPs) are essential regulators of gene expression, controlling mRNA splicing, stability, and non-coding RNA processing (miRNAs/lncRNAs). Dysregulation of these pathways drives numerous diseases, making RBP validation vital for functional genomics and target discovery.
The Electrophoretic Mobility Shift Assay (EMSA) is the gold standard for verifying direct RBP-RNA interactions. Running complexes on a native gel provides clear visual validation of binding specificity, affinity, and multi-protein assembly.
Principle
The assay is based on the fact that RNA-protein complexes migrate more slowly through a non-denaturing polyacrylamide gel than free RNA probes.
-
Binding Reaction: A biotin-labeled RNA probe is incubated with a purified protein or nuclear/cytoplasmic extract.
-
Electrophoresis: The mixture is resolved on a native gel. Free probes migrate quickly to the bottom, while bound RNA-protein complexes are retarded ("shifted") higher in the gel.
-
Detection: The gel is transferred to a membrane and visualized using an optimized, ultra-sensitive chemiluminescent substrate.
-
Specificity Control: Specificity is confirmed by adding an excess of unlabeled ("cold") competitor probe, which prevents the formation of the shifted biotin-labeled complex.
RNA EMSA Kits
Name | SKU | Price (USD) |
|---|---|---|
HuR (ELAVL1) RNA EMSA Kit | GR-0001 | $749.00 |
AUF1 (HNRNPD) RNA EMSA Kit | GR-0005 | $749.00 |
TTP (ZFP36) RNA EMSA Kit | GR-0008 | $749.00 |
CELF1 (CUGBP1) RNA EMSA Kit | GR-0009 | $749.00 |
IRP1 RNA EMSA Kit (IRE) | GR-0010 | $749.00 |
SLBP RNA EMSA Kit | GR-0011 | $749.00 |
IRP2 RNA EMSA Kit (IRE) | GR-0012 | $749.00 |
HCV IRES–eIF3 RNA EMSA Kit | GR-0013 | $749.00 |
PTBP1 (hnRNP I) RNA EMSA Kit | GR-0014 | $749.00 |
TDP-43 (TARDBP) RNA EMSA Kit | GR-0015 | $749.00 |
SRSF1 (ASF/SF2) RNA EMSA Kit | GR-0016 | $749.00 |
NOVA1/NOVA2 RNA EMSA Kit | GR-0017 | $749.00 |
LIN28A RNA EMSA Kit (let-7 Loop) | GR-0018 | $749.00 |
Staufen RNA EMSA Kit | GR-0019 | $749.00 |
Smaug RNA EMSA Kit | GR-0020 | $749.00 |
Pumilio (PUM1/2) RNA EMSA Kit | GR-0021 | $749.00 |
Benefits
Universal Compatibility
Validated for human, mouse, rat, and all other mammalian samples—ideal for translational research.
Safe, Non-Radioactive
Matches traditional radioactive sensitivity while eliminating regulatory burdens, hazards, and disposal costs.
Pre-Validated
Probe Pairs
Includes a biotin-labeled "hot" probe and an unlabeled "cold" competitor, enabling immediate specificity controls without oligo design.
Comprehensive Insights
Easily characterize binding dynamics, measure competition, and visualize multi-protein supershifts.
Ultra-Sensitive Chemiluminescence
Combining an optimized workflow with a highly sensitive substrate ensures clear detection of low-abundance RBPs and weak interactions.