Obesity increases the risk for the metabolic syndrome, diabetes, hypertension, atherosclerosis, and thrombosis. A number of proteins have been identified to be relevant to the development of the metabolic syndrome, diabetes, and cardiovascular disease with obesity. Plasma concentrations of these proteins are usually measured by ELISA. To systematically examine the effects, Signosis developed an ELISA strip which allows simultaneous determination of 8 obesity associated cytokines. The difference of these proteins between two samples can be determined through data comparison. Therefore, it facilitates the discovery of the change of these proteins in different samples.