Gene expression is regulated by individual cells, so why not study gene expression in a single cell such as stem cells, neurons, developmental tissues, and laser capture microsections. To allow researchers the ability to take on single cell studies, Signosis has developed a single cell SYBR Green based real-time RT-PCR Assay kit which allows you to directly assay gene expression levels in a single cell using cell lysate without any RNA preparation. This kit provides a simple, sensitive, quantitative and cost-effective method to monitor PCR reactions in real time. The reagents for cDNA synthesis and PCR amplification in addition to Direct cDNA cell lysis buffer are also included in the kit. Benefits: All-in-One - All reagents for the experiment, from cell lysis, reverse transcription to PCR assay, are included in one kit. No need for RNA preparation - Cell lysates can be used directly in reverse transcription. Robust - Full length cDNA can be obtained with the kit. Real-time - Analysis can be monitored in real time.