Knockout-TF Reporter Combo System

CRISPR Knockout
TF Reporter Combo System

Signosis offers over 80 stable TF reporter cell lines, including reporters for NRF2, NFκB, p53, SMAD1/5/8, and others, alongside a comprehensive suite of CRISPR knockout plasmids targeting pathway regulators, kinases, and receptors.

Principle

The Knockout-TF Reporter Combo System is a modular, ready-to-use assay platform that integrates CRISPR-mediated gene knockout with transcription factor (TF)-responsive luciferase reporter cell lines. Designed for pathway discovery, compound screening, and functional genomics, this system allows precise dissection of signaling networks by linking upstream gene perturbation to quantitative downstream TF activity.

Researchers can combine specific TF reporter lines with gene-specific CRISPR knockout plasmids to functionally interrogate pathway relationships.

For example:

NRF2 Reporter Cell Line + NRF2 Knockout: Decrease in luciferase signal under both basal and induced conditions.
NRF2 Reporter Cell Line + KEAP1 Knockout: Increased basal and induced luciferase activity due to constitutive activation of NRF2

Pathway Mapping and Deconvolution

Understanding how signaling pathways are regulated at the transcriptional level is critical for basic and translational research. By combining targeted CRISPR knockouts with transcription factor (TF) luciferase reporter cell lines, researchers can systematically interrogate the functional impact of individual genes on pathway activity. Knockouts of TFs (e.g., NRF2) or their regulators (e.g., KEAP1) allow users to distinguish upstream activators, repressors, and indirect modulators within complex signaling cascades.

Molecular Application
Slideshow

Product Pathways

All Products

Name	SKU	Price (USD)

NRF2 Pathway

The NRF2 pathway is a key regulator of the cellular response to oxidative stress. Under normal conditions, NRF2 is kept inactive in the cytoplasm by its inhibitor, KEAP1. However, in response to oxidative stress, NRF2 dissociates from KEAP1, translocates to the nucleus, and activates the expression of antioxidant genes, including HO-1, NQO1, and GCLC. This activation helps protect cells from damage caused by reactive oxygen species (ROS) and other stressors.

Related Cell Lines and CRISPR KO Plasmids:

NRF2 Reporter Cell Line: Measures NRF2 activity in response to oxidative stress.
NRF2 Knockout Plasmid: Knocks out NRF2 and study the resulting effects on gene expression and stress response.
KEAP1 Knockout Plasmid: Increases NRF2 activity by preventing its degradation, making it ideal for studying constitutive NRF2 activation.

Name	SKU	Formatted Price

See More NRF2 Reporter Cell Lines

See our TF Reporter Vectors

Wnt/β-Catenin Pathway

The Wnt/β-catenin signaling pathway is a fundamental regulator of cell proliferation, differentiation, and stem cell maintenance. In the absence of Wnt signals, β-catenin is continuously degraded by a destruction complex composed of AXIN1, APC, and GSK3β. When Wnt ligands bind their receptors, this complex is disrupted, allowing β-catenin to accumulate and translocate to the nucleus. There, it interacts with TCF/LEF transcription factors to drive expression of Wnt target genes.

Related Cell Lines and CRISPR KO Plasmids:

TCF/LEF Reporter Cell Line: Measures Wnt/β-catenin pathway activity via TCF/LEF-driven transcription.
β-Catenin (CTNNB1) Knockout Plasmid: Disrupts β-catenin expression to inhibit Wnt signaling.
AXIN1 Knockout Plasmid: Enhances β-catenin stability by disrupting the degradation complex, resulting in constitutive Wnt pathway activation.

Name	SKU	Formatted Price

See More TCF/LEF Reporter Cell Lines

See our TF Reporter Vectors

p53 / DNA Damage Response Pathway

The p53 pathway is a central regulator of the cellular response to DNA damage and other stress signals. Acting as a tumor suppressor, p53 promotes cell cycle arrest, apoptosis, or senescence in response to genotoxic stress. Under normal conditions, p53 levels are kept low by MDM2, an E3 ubiquitin ligase that targets p53 for degradation. When MDM2 is inhibited or lost, p53 is stabilized, allowing it to activate transcription of genes involved in DNA repair, cell death, and growth control.

Related Cell Lines and CRISPR KO Plasmids:

p53 Reporter Cell Line: Measures p53 activity in response to DNA damage or pathway modulation.
p53 (TP53) Knockout Plasmid: Eliminates p53 function to study its role in tumor suppression, DNA damage response, and therapeutic resistance.
MDM2 Knockout Plasmid: Disrupts the main negative regulator of p53, resulting in pathway activation — useful for studying p53-driven gene expression and sensitization to stress.

Name	SKU	Formatted Price

See More p53 Reporter Cell Lines

See our TF Reporter Vectors

NF-κB / Inflammation Pathway

The NF-κB pathway is a key regulator of inflammation, immune responses, and cell survival. In resting cells, NF-κB transcription factors—most notably the p65 (RELA) subunit—are held inactive in the cytoplasm by IκB proteins, such as IκBα (encoded by NFKBIA). Upon stimulation by inflammatory signals (e.g., TNFα, IL-1β, or LPS), IκBα is phosphorylated and degraded, freeing NF-κB to enter the nucleus and activate genes involved in cytokine production, immune regulation, and stress response.

Related Cell Lines and CRISPR KO Plasmids:

NF-κB Reporter Cell Line: Measures NF-κB activity in response to inflammatory stimuli.
p65 (RELA) Knockout Plasmid: Disrupts the key transcriptional subunit of NF-κB, silencing downstream target gene expression and inflammatory responses.
NFKBIA (IκBα) Knockout Plasmid: Removes the inhibitory control of NF-κB, resulting in constitutive activation — ideal for studying chronic NF-κB signaling and its downstream effects.

Name	SKU	Formatted Price

See More NRF2 Reporter Cell Lines

See our TF Reporter Vectors

NRF2 Pathway

The NRF2 pathway is a key regulator of the cellular response to oxidative stress. Under normal conditions, NRF2 is kept inactive in the cytoplasm by its inhibitor, KEAP1. However, in response to oxidative stress, NRF2 dissociates from KEAP1, translocates to the nucleus, and activates the expression of antioxidant genes, including HO-1, NQO1, and GCLC. This activation helps protect cells from damage caused by reactive oxygen species (ROS) and other stressors.

Related Cell Lines and CRISPR KO Plasmids:

NRF2 Reporter Cell Line: Measures NRF2 activity in response to oxidative stress.
NRF2 Knockout Plasmid: Used to delete NRF2 and study the resulting effects on gene expression and stress response.
KEAP1 Knockout Plasmid: Increases NRF2 activity by preventing its degradation, making it ideal for studying constitutive NRF2 activation.

Name	SKU	Formatted Price

See More NRF2 Reporter Cell Lines

See our TF Reporter Vectors

Benefits

Pathway Discovery, Simplified

Easily map signaling networks by pairing knockout plasmids with reporter lines for key transcription factors like NRF2, NFκB, p53, SMAD1/5/8, and more.

Precision-Driven Gene Functionality

Provides precise, gene-specific insights by linking CRISPR-induced knockouts to direct measurement of TF activity, revealing the impact of specific genes on cellular signaling.

Seamless Reporter Integration

Easily integrate TF reporters with gene knockouts to study how upstream genetic changes affect transcriptional outputs, enabling rapid mechanistic insights with minimal setup.

One Powerful System

Combines CRISPR knockout and TF-responsive reporters in one modular system to directly link gene disruption with transcriptional outcomes.

Accelerated Drug Mechanism Studies

Uncover molecular mechanisms behind drug efficacy or resistance by correlating knockout-induced changes in TF activity with drug treatment responses.

Precise Functional Genomics

Gain accurate, high-resolution insights into the functional roles of genes in transcriptional regulation, facilitating more targeted investigations in your research.

Ready-to-Use

Ready for immediate use, reducing setup time and enhancing experimental throughput in pathway discovery and functional genomics.

Flexible Experimental Design

Customize experiments, pairing different knockout plasmids with various TF reporters, making it ideal for diverse research goals, from basic biology to drug discovery.

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Quick Lead Time | Global Overnight Delivery Available

Purchase Order: info@signosisinc.com

CRISPR Knockout
TF Reporter Combo System

Principle

Molecular Application
Slideshow

Product Pathways

All Products

NRF2 Pathway

Wnt/β-Catenin Pathway

p53 / DNA Damage Response Pathway

NF-κB / Inflammation Pathway

NRF2 Pathway

Benefits

Related Products

Stable Cell Line

TF Assays

Cytokine

Biomarkers

Quick Lead Time | Global Overnight Delivery Available

Leading Bioassay Provider Globally

Quick Lead Time | Global Overnight Delivery Available

Purchase Order: info@signosisinc.com

​CRISPR Knockout TF Reporter Combo System

Principle

Molecular Application Slideshow

Product Pathways

All Products

Benefits

Related Products

Stable Cell Line

TF Assays

Cytokine

Biomarkers

Quick Lead Time | Global Overnight Delivery Available

Leading Bioassay Provider Globally

CRISPR Knockout
TF Reporter Combo System

Molecular Application
Slideshow