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​CRISPR Knockout
TF Reporter Combo System

Signosis offers over 80 stable TF reporter cell lines, including reporters for NRF2, NFκB, p53, SMAD1/5/8, and others, alongside a comprehensive suite of CRISPR knockout plasmids targeting pathway regulators, kinases, and receptors.

Principle 

The Knockout-TF Reporter Combo System is a modular, ready-to-use assay platform that integrates CRISPR-mediated gene knockout with transcription factor (TF)-responsive luciferase reporter cell lines. Designed for pathway discovery, compound screening, and functional genomics, this system allows precise dissection of signaling networks by linking upstream gene perturbation to quantitative downstream TF activity.

Researchers can combine specific TF reporter lines with gene-specific CRISPR knockout plasmids to functionally interrogate pathway relationships.

 

For example:

  • NRF2 Reporter Cell Line + NRF2 Knockout: Decrease in luciferase signal under both basal and induced conditions.

  • NRF2 Reporter Cell Line + KEAP1 Knockout: Increased basal and induced luciferase activity due to constitutive activation of NRF2

Keap1–Nrf2 Pathway.png

Pathway Mapping and Deconvolution

Understanding how signaling pathways are regulated at the transcriptional level is critical for basic and translational research. By combining targeted CRISPR knockouts with transcription factor (TF) luciferase reporter cell lines, researchers can systematically interrogate the functional impact of individual genes on pathway activity. Knockouts of TFs (e.g., NRF2) or their regulators (e.g., KEAP1) allow users to distinguish upstream activators, repressors, and indirect modulators within complex signaling cascades.

Molecular Application
Slideshow

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Benefits

Pathway Discovery, Simplified

Easily map signaling networks by pairing knockout plasmids with reporter lines for key transcription factors like NRF2, NFκB, p53, SMAD1/5/8, and more.

Precision-Driven Gene Functionality

Provides precise, gene-specific insights by linking CRISPR-induced knockouts to direct measurement of TF activity, revealing the impact of specific genes on cellular signaling.

Seamless Reporter Integration

Easily integrate TF reporters with gene knockouts to study how upstream genetic changes affect transcriptional outputs, enabling rapid mechanistic insights with minimal setup.

One Powerful System

Combines CRISPR knockout and TF-responsive reporters in one modular system to directly link gene disruption with transcriptional outcomes.

Accelerated Drug Mechanism Studies

Uncover molecular mechanisms behind drug efficacy or resistance by correlating knockout-induced changes in TF activity with drug treatment responses.

Precise Functional Genomics

Gain accurate, high-resolution insights into the functional roles of genes in transcriptional regulation, facilitating more targeted investigations in your research.

Ready-to-Use

Ready for immediate use, reducing setup time and enhancing experimental throughput in pathway discovery and functional genomics.

Flexible Experimental Design

Customize experiments, pairing different knockout plasmids with various TF reporters, making it ideal for diverse research goals, from basic biology to drug discovery.

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