TF Luciferase Reporter Vectors
Quantify cellular responses with high sensitivity using Signosis Transcription Factor (TF) Reporter Vectors.
Precision Monitoring of Gene Transcription
Signosis TF Luciferase Reporter Vectors are specifically engineered to monitor the initiation and regulation of transcription. By utilizing these high-sensitivity tools, researchers can precisely measure how specific factors drive the transcription of a luciferase reporter gene in response to cellular signals.
This provides a robust platform for studying transcription-level regulation within signal transduction pathways, offering the flexibility of built-in normalization or the generation of stable cell lines for consistent, high-precision data.
Activated transcription factors bind to cis-regulatory elements upstream of the luciferase reporter gene, triggering luciferase expression detectable in reporter assays.
Choose Your Reporter System
1. Transient Transfection (No Selection Marker)
For short-term studies (24–72 hours post-transfection), these vectors are streamlined for high expression without the overhead of antibiotic resistance genes.
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Basic Firefly or Renilla Luciferase Vectors: Provides a simple, reliable chemiluminescent readout of TF activation.
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Firefly–Renilla Dual Vectors: The gold standard for accuracy. This "two-in-one" vector contains both the TF-driven Firefly luciferase and a constitutive CMV-driven Renilla luciferase to normalize for transfection efficiency in every well.
2. Stable Cell Line Generation (with Selectable Markers)
For long-term monitoring and reproducible screening, these vectors include selection markers to generate permanent reporter cell lines.
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HygroSelect & NeoSelect: Available with Hygromycin or Neomycin resistance.
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Visual Selection: Features integrated GFP for easy identification of successfully transfected colonies.
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Dual-Pathway Monitoring: Co-transfect one HygroSelect and one NeoSelect vector to monitor two different pathways simultaneously in the same cell line.
Key Benefits
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High Sensitivity: Engineered with ≥ 4 copies of enhancer elements for maximum signal induction.
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Low Background: Minimal TATA promoter ensures high induction-to-basal ratios.
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Massive Selection: Over 100 validated TF targets available off-the-shelf.
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Application-Specific: Optimized transient vectors (no selection marker) or stable cell line vectors (Hygro/Neo/GFP).
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Total Solution: Fully compatible with Signosis high-sensitivity substrates and custom development services.
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Proven Reliability: Trusted by leading researchers at Harvard, NIH, Bristol Myers Squibb, and hundreds of other academic and pharmaceutical institutions worldwide.
Principle
Signosis reporter vectors quantify TF activity through a straightforward four-step process:
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Enhancer Binding: Activated TFs bind to specific cis-acting enhancer elements upstream of a minimal TATA promoter.
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Gene Expression: Binding triggers the transcription of the Firefly or Renilla luciferase reporter gene.
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Signal Generation: Luciferase protein reacts with added substrate to produce a chemiluminescent signal.
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Quantification: The intensity of light directly corresponds to the level of transcription factor activation.
Product Categories
Associated Reagents & Substrates
To ensure the highest signal-to-noise ratio, use our ready-to-use substrates specifically formulated for these vector systems:
Custom Vector Services
With over 100 vectors in stock, we cover most major pathways. If you require a specific transcription factor, unique enhancer sequence, or custom selection marker, Signosis offers:
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Custom Vector Construction
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Custom Stable Cell Line Development
Contact us for a quote or technical recommendation:
Phone: 408-747-0771 Email: info@signosisinc.com
Stable Cell Line Development
Our HygroSelect and NeoSelect vectors are designed for researchers needing long-term, reproducible data without the variability of transient transfections.
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Dual-Pathway Monitoring: By using one HygroSelect vector and one NeoSelect vector together, you can monitor two different signaling pathways within the same cell line.
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Visual Selection: Integrated GFP markers allow for easy monitoring of transfection efficiency and colony selection.
We can also provide ready made Luciferase Reporter Stable Cell lines using these reporter vectors.
Experimental Design Notes
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Baselines: For most high-throughput assays or relative-induction experiments, untreated wells using the pTA-TF vector serve as a sufficient baseline.
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Basal Activity: If your research requires the precise measurement of basal promoter activity, we recommend including a pTA-control vector in your experimental design.
