Culture-Free Cancer Cell Panel Assay
Introduction
Signosis' Culture-Free Cancer Cell Panel Assay is a ready-to-run 96-well assay plate pre-seeded with validated luciferase reporter stable cell lines targeting eight critical oncogenic and cancer-related transcription factor (TF) pathways: MYC, E2F, HIF, p53, STAT3, AP-1, NFκB, and CREB.
Designed for cancer research, drug screening, and tumor microenvironment profiling, this innovative platform eliminates the need for cell seeding or culture maintenance. Simply add your compounds directly to the wells and measure luciferase activity to gain fast, reproducible insights into tumor progression, cell cycle regulation, hypoxia response, and inflammatory signaling.
Explore our full collection of transcription factor (TF) luciferase reporter stable cell lines, including those used in the panel and additional TFs available for individual purchase. These cryopreserved cell lines can be cultured using standard techniques, offering flexibility for custom assay development and mechanistic studies.
Custom configurations are also available to include other TFs of interest, providing a flexible and standardized solution for oncogenic and cancer-related drug discovery and mechanistic research.
Principle
The One-Step Oncogenic & Cancer Pathway Reporter Panel utilizes luciferase reporter stable cell lines to monitor the activation of eight transcription factor (TF) pathways central to tumor biology, drug response, and cancer progression. Each reporter cell line contains a luciferase gene driven by TF-specific response elements. When activated, the TF binds its response element, inducing luciferase expression, which is quantified as luminescence and reflects pathway activity.
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MYC: Monitors transcriptional activity linked to cell growth, proliferation, and metabolic reprogramming in cancer.
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E2F: Detects cell cycle–related transcriptional activation involved in DNA replication and tumor progression.
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HIF (Hypoxia-Inducible Factor): Reports on hypoxia signaling and tumor microenvironment adaptation that drive angiogenesis and metabolic shifts.
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p53: Tracks DNA damage response, cell cycle arrest, and apoptosis regulation in cancer cells.
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STAT3: Reflects cytokine and growth factor signaling associated with tumor cell survival, immune evasion, and metastasis.
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AP-1 (Activator Protein 1): Monitors stress- and growth factor–driven transcription linked to proliferation, transformation, and invasion.
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NFκB (Nuclear Factor Kappa B): Detects inflammatory and pro-survival signaling that contribute to cancer progression and therapy resistance.
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CREB (cAMP Response Element-Binding Protein): Reports on transcriptional programs that regulate tumor cell growth, metabolism, and survival.
Application
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Drug Resistance Research: Investigate mechanisms of therapy resistance and identify strategies to overcome treatment failure.
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DNA Damage Response Studies: Analyze pathway activation involved in DNA repair, checkpoint control, and genomic stability.
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Tumor Suppressor Profiling: Characterize the functional role of tumor suppressors such as p53 in regulating oncogenic signaling.
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Cancer Drug Screening: Evaluate compound effects on oncogenic and tumor-suppressor pathways in a single assay format.
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Precision Oncology Research: Explore pathway-specific responses to support targeted cancer therapy development.
Benefits
No Culture Required
Pre‑seeded with luciferase reporter cell lines, ready for immediate testing
Standardized & Reproducible
Consistent cell seeding and validation ensure reliable comparisons
Complete Reagent Package
Includes lysis buffer and luciferase substrate
Covers Eight Core Stress Pathways
Simultaneously analyze 8 key transcription factor (TF) pathways in a single 96-well format for efficient pathway screening.
Highly Sensitive & Quantitative
Direct correlation between TF activation and luminescence signal
Customizable
Option to include additional transcription factors relevant to your research
Fast, One‑Step Workflow
Add compounds and measure luminescence, reducing hands‑on time
Ideal for Multiple Applications
Perfect for antioxidant drug screening, toxicology, stress biology, and mechanistic studies