Oxidative Assay Kit
Using hydrogen peroxide to induce oxidative stress in cellular models is a common practice, but the doses used are much higher than physiological oxygen levels. This necessitates a need for a precise and sensitive method to measure hydrogen peroxide in various samples.
Principle
The Hydrogen Peroxide (H2O2) Assay kit utilizes the Amplex Red reagent and horseradish peroxidase (HRP) to detect H2O2 in biological samples, such as cells and enzyme reactions. When combined with HRP, the Amplex Red reagent reacts with H2O2 to produce resorufin, a fluorescent red oxidation product. Resorufin has an excitation range of 530-560 nm and can be detected at a fluorescence emission wavelength of 590 nm. Alternatively, the signal can also be read spectrophotometrically at an absorbance of 560 nm.
Benefits
Flexible
Quantify hydrogen peroxide in solution, in cell extracts or directly from certain cells
Versatile
Detect a variety of oxidase activities through enzyme-coupled reactions
Sensitive
Detect as little as 10 picomoles of hydrogen peroxide
Complete
Mix and read reagents, with minimal hands-on time
Convenient
Homogenous assay format, fully compatible with HTS automation