The Oxidative & ER Stress Response Panel utilizes luciferase reporter stable cell lines to monitor the activation of eight key transcription factor (TF) pathways involved in oxidative stress, ER stress, hypoxia, DNA damage, and inflammatory responses. Each reporter cell line contains a luciferase gene under the control of TF-specific response elements. When the corresponding TF is activated, it binds to its response element, driving luciferase expression that can be quantified as luminescence.

​

• NRF2 (Nuclear Factor Erythroid 2–Related Factor 2): Detects antioxidant response element (ARE) activation, a central mechanism for cellular defense against oxidative stress and electrophilic insults.

• CHOP (C/EBP Homologous Protein): Monitors ER stress–induced apoptosis via the unfolded protein response (UPR) pathway, serving as a key marker of prolonged or severe ER stress.

• ATF4 (Activating Transcription Factor 4): Reports on the PERK branch of the UPR, linked to amino acid metabolism, redox balance, and stress adaptation.

• ATF6 (Activating Transcription Factor 6): Detects ER stress signaling through ATF6 activation, which regulates chaperone expression and ER-associated protein folding.

• HIF (Hypoxia-Inducible Factor): Monitors cellular response to low oxygen levels, enabling profiling of hypoxia-related signaling pathways in metabolic and stress-related contexts.

• p53: Reports on DNA damage and genotoxic stress, reflecting the activation of p53-dependent transcriptional programs controlling cell cycle arrest and apoptosis.

• NFκB (Nuclear Factor Kappa B): Detects inflammatory and stress signaling related to oxidative damage, cytokine production, and immune responses.

• CREB (cAMP Response Element-Binding Protein): Monitors cAMP-dependent signaling that connects energy metabolism, stress adaptation, and cell survival pathways.