CRISPR Knock-in
HDR Repair Vectors
Signosis CRISPR HDR Repair-Ready Vectors enable precise knock‑ins and sequence corrections using a flexible two‑vector system. Pair our Cas9 Knockout Vector with our CRISPR HDR Knock-in Vectors, available with prebuilt safe‑harbor donors (mROSA26, hAAVS1) or fully customized templates for any genomic locus.
Principle
Signosis' CRISPR Homology-Directed Repair (HDR) Repair System delivers accurate, template‑driven genome editing through a streamlined two‑vector workflow. A dedicated Cas9 Knockout Vector creates a targeted double‑strand break, while a separate HDR knock-in Vector supplies either a prebuilt or fully customized donor template for precise knock‑ins, tag additions, or sequence corrections. This modular design provides maximum editing flexibility, enabling rapid use of validated safe‑harbor donors (mROSA26 and hAAVS1) while supporting custom HDR designs for any gene or genomic site. Ideal for reporter knock‑ins, protein tagging, functional rescue experiments, and targeted gene correction.
CRISPR HDR gene editing is a two‑step process:
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Cas9 Knockout Vector (KO Vector)
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Expresses Cas9 and a locus‑specific gRNA
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Creates the necessary DNA break at the target site
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HDR knock-in Vector:
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Delivers a donor template with homology arms
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Guides precise DNA repair via homology‑directed repair
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By separating the cut and repair functions into two vectors, the system maximizes editing efficiency, reduces vector size constraints, and supports a wider range of donor template configurations.
Key Features
Validated, Ready-to-use Safe‑Harbor Donor Templates targeting:
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mROSA26 (mouse)
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hAAVS1 (human)
Ideal for stable expression, reporter integration, and predictable knock‑ins.
Two‑Vector CRISPR knock-in HDR Workflow
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A modular system separating Cas9/gRNA knockout from HDR donor delivery, improving flexibility and transfection efficiency.
Fully Custom Donor Template Capability
Design knockout‑compatible donor plasmids for:
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Any gene or genomic locus
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SNP corrections
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Epitope or fluorescent tags
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Functional domains
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Regulatory motifs
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Sequence replacements
All‑in‑One Constructs for Each Step
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KO Vector includes Cas9 + gRNA
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HDR knock-in vector contains donor template + homology arms
Optimized Homology Arms
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Balanced arm lengths and sequence design for high HDR efficiency.
Broad Application Compatibility
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Supports use in multiple cell lines, including hard‑to‑edit systems.
Benefits
High-precision
Crispr technology allows clean, precise gene insertion via HDR-based editing
Reliable, Consistent Performance
Standardized backbones across all vectors allow for consistent results
Modular 2‑vector system
Mix and Match between different HDR vectors for target customization
Easy Transfection
One vector for each part of the process simplifies delivery and reduces variability
Fast and Efficient workflows
Use prebuilt safe-harbor donors templates to ensure stable, predictable, non-disruptive knock-ins.
Customizable
Both sgRNAs and donor templates can be tailored to your target of interest
Products
Target | Name | SKU | Price (USD$) |
|---|---|---|---|
NRF2/ARE | NRF2 KO Vector | KO-0001 | $800.00 |
NRF2/ARE | Keap1 KO Vector | KO-0002 | $800.00 |
NFkB | NFKBIA (IκBα) KO Vector | KO-0003 | $800.00 |
NFkB | p65 KO Vector | KO-0004 | $800.00 |
p53 | MDM2 KO Vector | KO-0005 | $800.00 |
p53 | p53 KO Vector | KO-0006 | $800.00 |
ER Stress-ERSE | PERK/EIF2AK3 KO Vector | KO-0007 | $800.00 |
ER Stress-ERSE | IRE1/ERN1 KO Vector | KO-0008 | $800.00 |
ER Stress-ERSE | ATF6 KO Vector | KO-0009 | $800.00 |
SMAD/BMP | Smad1/5/8 KO Vectors | KO-0010 | $800.00 |
SMAD/BMP | BMPR1 KO Vector | KO-0011 | $800.00 |
SMAD/BMP | BMPR2 KO Vector | KO-0012 | $800.00 |
SMAD/BMP | Smad4 KO Vector | KO-0013 | $800.00 |
SMAD/TGFbeta | TGFBR2 KO Vector | KO-0014 | $800.00 |
SMAD/TGFbeta | Smad2/3 KO Vectors | KO-0015 | $800.00 |
SMAD/TGFbeta | Smad7 KO Vectors | KO-0016 | $800.00 |
TCF/LEF | β-catenin KO Vector | KO-0017 | $800.00 |
TCF/LEF | AXIN1 KO Vector | KO-0018 | $800.00 |
Control | FLUC Positive Control KO Vector | KO-C001 | $800.00 |
Control | GLUC Positive Control KO Vector | KO-C002 | $800.00 |
Control | Non-Targetting Negative Control KO Vector | KO-C003 | $800.00 |
Control | hAASV1 Negative Control KO Vector | KO-C004 | $800.00 |
null | Custom KO Vector | cus-KO-0001 | $900.00 |