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Isoform-Specific
Nuclear Hormone Receptor
Reporter Stable Cell Lines
Nuclear hormone receptors (NHRs) are ligand-activated transcription factors with conserved DNA-binding domains, making it challenging to distinguish individual members. To address this, Signosis developed Nuclear Hormone Receptor Reporter Stable Cell Lines, which use the GAL4 system fused with the ligand-binding domains (LBD) of specific nuclear hormone receptors to enable precise receptor-specific activation analysis. These lines cover receptors such as PPARα, PPARγ, PPARδ, GRα, and PXR.
Principle
Traditional luciferase reporter cell lines often lack specificity due to the conserved DNA-binding motifs among nuclear hormone receptors (NHRs). To overcome this, Signosis developed NHR LBD-driven GAL4 reporter cell lines using a dual-vector system. One vector encodes a GAL4 DNA-binding domain fused to the ligand-binding domain (LBD) of a specific NHR, while the other contains eight GAL4 UAS sites upstream of a luciferase reporter. Upon ligand binding, the chimeric DBD-LBD protein activates luciferase expression, yielding a specific signal with minimal cross-reactivity. The system shows low cellular toxicity and high sensitivity. Stable clones were selected using dual antibiotics (hygromycin and neomycin) and validated for strong, ligand-dependent induction, making them ideal for screening NHR modulators.
Nuclear Hormone Receptor
Stable Cell Lines Products
GAL4-UAS-Luc Cell Lines Products
Signosis has developed the GAL4-UAS-Luc Reporter Stable Cell Lines as a reliable, rapid, and sensitive method for analyzing the expression of your target gene of interest upon exposure to related ligands or other stimuli. These cell lines are transfected only with the GAL4-UAS-LUC vector, and are ready to be transfected with your customized GAL4 vector containing your NR of interest.
Name | SKU | Formatted Price |
|---|---|---|
UAS Luciferase Reporter CHO Stable Cell Line | SL-5000 | $2,000.00 |
UAS Luciferase Reporter HEK293 Stable Cell Line | SL-3000 | $2,000.00 |
UAS Luciferase Reporter HeLa Stable Cell Line | SL-4000 | $2,000.00 |
Benefits
Highly Specific
The responsiveness of this stable cell line is driven by the specific ligand-binding domain with low/no cross-reactivity with other members of the nuclear receptor.
High Dynamic Range Response
More than 30-fold induction in response to the corresponding stimuli.
We offer a highly sensitive Firefly Luciferase Substrate, which can accurately measure firefly luciferase activity in cells.
Mycoplasma Free
All cell lines have been tested negative for mycoplasma.
Ultra-Sensitive
Delivers Robust Response Even at 1 nM.
Research Application
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Studying the effects of ligand binding on GAL4-mediated transcriptional activity
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Identifying ligands that activate or inhibit the target receptors by monitoring GAL4-driven luciferase expression
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Screening for potential therapeutic compounds that modulate the target receptors by measuring changes in luciferase activity
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Investigating the mechanisms underlying the regulation of the target receptors by analyzing the GAL4-mediated transcriptional response to various stimuli
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Comparing the activity and selectivity of different compounds on the target receptors by using the GAL4 reporter assay
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Studying the interaction between the target receptors and other signaling pathways or co-regulators by analyzing the changes in GAL4-mediated transcriptional activity upon co-expression of different genes or treatments.
