Toll Free 1-877-266-8388 Tel: 1-408-747-0771
Fax: 1-408-470-7719
Quick Lead Time | Global Overnight Delivery Available
Purchase Order: info@signosisinc.com
Isoform-Specific
Nuclear Hormone Receptor
Reporter Stable Cell Lines
Nuclear hormone receptors (NHRs) share structurally conserved DNA-binding domains, which can lead to overlapping recognition of response elements. Their functional specificity is further complicated by similarities in ligand-binding domains (LBDs), shared co-regulators, and common dimerization partners. To address these challenges, Signosis developed Nuclear Hormone Receptor Reporter Stable Cell Lines that utilize the GAL4 system, in which the GAL4 DNA-binding domain is fused to the LBD of a specific NHR. This design enables precise, receptor-specific activation analysis. These reporter lines cover receptors such as PPARα, PPARγ, PPARδ, GRα, and PXR.
Principle
Traditional luciferase reporter cell lines often lack specificity due to the conserved DNA-binding motifs among nuclear hormone receptors (NHRs). To overcome this, Signosis developed NHR LBD-driven GAL4 reporter cell lines using a dual-vector system. One vector encodes a GAL4 DNA-binding domain fused to the ligand-binding domain (LBD) of a specific NHR, while the other contains eight GAL4 UAS sites upstream of a luciferase reporter. Upon ligand binding, the chimeric DBD-LBD protein activates luciferase expression, yielding a specific signal with minimal cross-reactivity. The system shows low cellular toxicity and high sensitivity. Stable clones were selected using dual antibiotics (hygromycin and neomycin) and validated for strong, ligand-dependent induction, making them ideal for screening NHR modulators.
Nuclear Hormone Receptor
Stable Cell Lines Products
Name | SKU | Price (USD) |
|---|
GAL4-UAS-Luc Cell Lines Products
Signosis has developed the GAL4-UAS-Luc Reporter Stable Cell Lines as a reliable, rapid, and sensitive method for analyzing the expression of your target gene of interest upon exposure to related ligands or other stimuli. These cell lines are transfected only with the GAL4-UAS-LUC vector, and are ready to be transfected with your customized GAL4 vector containing your NR of interest.
Name | SKU | Formatted Price |
|---|---|---|
UAS Luciferase Reporter CHO Stable Cell Line | SL-5000 | $2,000.00 |
UAS Luciferase Reporter HEK293 Stable Cell Line | SL-3000 | $2,000.00 |
UAS Luciferase Reporter HeLa Stable Cell Line | SL-4000 | $2,000.00 |
Benefits
Highly Specific
The responsiveness of this stable cell line is driven by the specific ligand-binding domain with low/no cross-reactivity with other members of the nuclear receptor.
High Dynamic Range Response
More than 30-fold induction in response to the corresponding stimuli.
We offer a highly sensitive Firefly Luciferase Substrate, which can accurately measure firefly luciferase activity in cells.
Mycoplasma Free
All cell lines have been tested negative for mycoplasma.
Ultra-Sensitive
Delivers Robust Response Even at 1 nM.
Research Application
-
Studying the effects of ligand binding on GAL4-mediated transcriptional activity
-
Identifying ligands that activate or inhibit the target receptors by monitoring GAL4-driven luciferase expression
-
Screening for potential therapeutic compounds that modulate the target receptors by measuring changes in luciferase activity
-
Investigating the mechanisms underlying the regulation of the target receptors by analyzing the GAL4-mediated transcriptional response to various stimuli
-
Comparing the activity and selectivity of different compounds on the target receptors by using the GAL4 reporter assay
-
Studying the interaction between the target receptors and other signaling pathways or co-regulators by analyzing the changes in GAL4-mediated transcriptional activity upon co-expression of different genes or treatments.
