The study of gene expression often needs RNA preparation followed by cDNA synthesis and PCR, but most of the time, you don't want to waste a large amount of cells for RNA preparation. This is a particular problem for researchers using laser dissected samples, FACS sorted cells, cultured cells in 96-wells, and liquid biopsy. Signosis� Direct cDNA cell lysis buffer allows researchers to prepare cell lysate from small samples, which can be used for direct reverse transcription without RNA preparation. Cell lysate made from even a few cells is good enough for reverse transcription of RNA to cDNA, which you can then use for PCR analysis or cDNA plate array assays.
 
Benefits:
Simplified procedures - Reverse transcription can be performed in cell lysate without RNA preparation.
Limited cell numbers required - A few cells are good enough to make cDNA for PCR.