RNA-Protein Interactome
Profiling Plate
A high-throughput, plate-based assay for simultaneous profiling of RNA–protein interactions across multiple regulatory pathways—delivering quantitative results in a single experiment.
RNA–protein interactions play a central role in post-transcriptional gene regulation, influencing processes such as mRNA stability, translation, alternative splicing, and miRNA biogenesis. Comprehensive analysis of RNA-binding protein (RBP) activity is therefore critical for understanding cellular function and disease mechanisms.
However, traditional methods such as RNA EMSA (Electrophoretic Mobility Shift Assay) are low-throughput, labor-intensive, and provide limited quantitative insight, restricting their use in large-scale or sample-limited studies.
Signosis' RNA-Protein Interactome Profiling Plate is designed to address these challenges by enabling multiplex, quantitative analysis of RNA–protein interactions in a convenient microplate format. This platform allows researchers to monitor 16 distinct RNA-binding activities simultaneously, providing a broader and more efficient view of RBP function using minimal sample input.
Principle
The Signosis RNA-Protein Interactome Profiling Plate utilizes a multiplex RNA-binding assay to detect and quantify interactions between RNA motifs and RNA-binding proteins in a microplate-based format.
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RNA Motif Binding
A panel of defined RNA oligonucleotides representing distinct regulatory motifs is incubated with the sample. RNA-binding proteins selectively associate with their corresponding target sequences. -
Complex Capture
RNA–protein complexes are immobilized onto the plate via affinity-based capture, separating bound from unbound components. -
Signal Development
Bound proteins are detected using antibody-based or universal detection chemistry, followed by enzymatic signal generation. -
Quantitative Readout
The resulting signal is measured using a standard microplate reader. Signal intensity corresponds to the relative binding activity for each RNA motif, enabling direct comparison across multiple interactions.
This workflow eliminates the need for electrophoresis and gel interpretation, providing a streamlined, scalable, and quantitative alternative to traditional EMSA-based approaches.
Products
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Benefits
Eliminate Tedious Gel Shifts (EMSAs)
Converts slow, low-throughput Electrophoretic Mobility Shift Assays (EMSAs) into a fast, gel-free microplate format.
Quantitative & Sensitive
Allows detection of binding strength and affinity using biotin–streptavidin chemiluminescence.
Comprehensive Profiling
Profiles 16 key regulatory RNA motifs in a single assay, mapping complex post-transcriptional networks at once.
Versatile Probe Design
In vitro transcription enables custom probe synthesis from functional regulatory regions.
High-Throughput Screen Ready
Optimized for rapid screening of drug compounds, inhibitors, or cytokines across multiple pathways in parallel.
No Specialized Infrastructure
Bypasses the need for expensive mass spectrometry or sequencing, reading out on a standard lab plate reader.
Physiologically Relevant Readouts
Detects endogenous RNA-binding protein (RBP) activity directly from crude cell or tissue lysates.
100%
Non-Radioactive
Uses biotin labeling for safe, stable, and chemiluminescent detection—no radioactive handling required.