16-Plex RNA–Protein
Interaction Plate Array
A high-throughput, plate-based assay for simultaneous profiling of 16 RNA–binding pathways in one assay.
RNA–protein interactions play a central role in post-transcriptional gene regulation, influencing processes such as mRNA stability, translation, alternative splicing, and miRNA biogenesis. Comprehensive analysis of RNA-binding protein (RBP) activity is therefore critical for understanding cellular function and disease mechanisms.
However, traditional methods such as RNA EMSA (Electrophoretic Mobility Shift Assay) are low-throughput, labor-intensive, and provide limited quantitative insight, restricting their use in large-scale or sample-limited studies.
Signosis' 16-Plex RNA–Protein Interaction Plate Array is designed to address these challenges by enabling multiplex, quantitative measurement of RNA–protein binding activity in a convenient microplate format. This platform allows researchers to monitor 16 distinct RNA-binding activities simultaneously, providing a broader and more efficient view of RBP function using minimal sample input.
Principle
The Signosis 16-Plex RNA–Protein Interaction Plate Array utilizes a multiplex RNA-binding assay to detect and quantify interactions between RNA motifs and RNA-binding proteins in a microplate-based format.
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RNA Motif Binding
A panel of defined RNA oligonucleotides representing distinct regulatory motifs is incubated with the sample. RNA-binding proteins selectively associate with their corresponding target sequences. -
Complex Capture
RNA–protein complexes are captured in the microplate via probe-based immobilization, allowing unbound components to be removed while preserving specific interactions. -
Signal Development
Bound RNA-proteins are detected via biotin-based chemiluminescent detection, generating a measurable signal proportional to binding activity. -
Quantitative Readout
The resulting signal is measured using a standard microplate reader. Signal intensity corresponds to the relative binding activity for each RNA motif, enabling direct comparison across multiple interactions.
This workflow eliminates the need for electrophoresis and gel interpretation, providing a streamlined, scalable, and quantitative alternative to traditional EMSA-based approaches.
Benefits
Replaces Tedious Gel Shifts (EMSAs)
Converts slow, low-throughput Electrophoretic Mobility Shift Assays (EMSAs) into a fast, gel-free microplate format.
Quantitative & Sensitive
Allows sensitive detection of relative RNA–protein binding activity using biotin–chemiluminescent readout.
Targeted
Profiling
Profiles 16 key regulatory RNA motifs in a single assay at once, covering key RBPs in disease, development, and RNA regulation.
Validated Probe Design
Pre-validated RNA probes targeting key regulatory motifs ensure consistent and reproducible results.
High-Throughput Screen Ready
96-well plate format enables parallel analysis of multiple samples, treatments, or compounds.
No Specialized Infrastructure
Bypasses the need for expensive mass spectrometry or sequencing via simple readout on a standard lab plate reader.
Physiologically Relevant Readouts
Measures endogenous RBP-binding activity directly from cell or tissue lysates, preserving native binding interactions.
100%
Non-Radioactive
Uses biotin labeling for safe, stable, and chemiluminescent detection—no radioactive handling required.