Lentiviral Expression Vectors
Introduction
Signosis Lentiviral Expression Vectors are transfer vectors designed to carry a gene of interest for stable and efficient delivery into mammalian cells. They are a key component of lentiviral transduction systems, enabling long-term gene expression in both dividing and non-dividing cells.
These vectors do not produce virus on their own; instead, they function in combination with packaging cells and helper plasmids to generate replication-deficient lentiviral particles. This modular design ensures safe laboratory handling while providing a robust platform for cellular engineering, including stable gene overexpression, reporter cell line generation, and functional genomics studies.
Principle
Lentiviral expression relies on the natural ability of lentiviruses to deliver genetic material into host cells and integrate it into the genome. In a typical system:
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The gene of interest is cloned into a transfer vector containing regulatory elements such as promoters and selection markers.
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The transfer vector is co-transfected into packaging cells (LentiPack 293T, SKU: PC-023) along with helper plasmids that supply essential viral proteins (gag, pol, rev, and envelope proteins) in trans.
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Viral particles are assembled, carrying only the gene of interest, and released into the culture medium.
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Target cells are transduced with these particles. Upon entry, the viral RNA is reverse-transcribed into DNA and integrated into the host genome.
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The inserted gene is stably expressed, inherited by daughter cells, and suitable for downstream applications such as drug screening, reporter assays, or functional studies.
This system combines high efficiency, broad cell-type compatibility, and long-term stability while maintaining safety and reproducibility in laboratory use.
Custom Lentiviral Expression Vector Service
Signosis also offers a custom lentiviral expression vector service to meet specific research needs:
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Delivery of any gene of interest into your choice of vector backbone.
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Custom promoters, selection markers, and reporter options.
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Co-expression of multiple genes or regulatory elements.
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Support for stable cell line generation with reproducible expression.
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Technical guidance for packaging, transduction, and assay optimization.
Our team works closely with clients to design, construct, and validate lentiviral vectors tailored for their unique experimental goals, enabling efficient and reliable gene delivery for research or therapeutic applications.
Key benefits
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Safe Laboratory Use: Replication-deficient design ensures safe handling in standard lab conditions.
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Flexible Customization: Support for any gene of interest, custom promoters, reporters, selection markers, and co-expression constructs.
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Versatile Applications: Ideal for stable cell line development, reporter assays, functional genomics, drug screening, and mechanistic studies.
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Secreted/Reporter Options: Non-destructive, real-time monitoring of gene expression or pathway activity is possible with secreted reporters.
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Reproducibility: Standardized protocols and validated constructs ensure consistent performance across experiments.
Products
Name | SKU | Formatted Price |
|---|---|---|
LentiPack 293T | PC-023 | $445.00 |
pLenti-EGFR (A767_dupASV, C797S) | ELM-0002 | $1,935.00 |
pLenti-EGFR (D770_N771insSVD, C797S) | ELM-0004 | $1,935.00 |
pLenti-EGFR (H773_V774iinsNPH) | ELM-0003 | $1,935.00 |
pLenti-EGFR (H773_V774insH, C797S) | ELM-0005 | $1,935.00 |
pLenti-EGFR (L718Q, L858R) | ELM-0006 | $1,935.00 |
pLenti-EGFR (L718Q, T790M, L858R) | ELM-0008 | $1,935.00 |
pLenti-EGFR (L792H, L858R) | ELM-0007 | $1,935.00 |
pLenti-EGFR (T790M, L792H, L858R) | ELM-0009 | $1,935.00 |
pLenti-EGFR (Viii) | ELM-0010 | $1,935.00 |
pLenti-EGFR (WT) | ELM-0001 | $1,571.00 |
pLenti-MSCV-EGFR-GFP-HYG Vector, EGFR (Del19), non-FLAG tagged | ELM-0011 | $1,935.00 |
pLenti-MSCV-EGFR-GFP-HYG Vector, EGFR (L858R), non-FLAG tagged | ELM-0012 | $1,935.00 |